纳米金刚石作为siRNA递送至尤文肉瘤细胞的载体
Nanodiamond as a vector for siRNA delivery to Ewing sarcoma cells
摘要：
我们研究了金刚石纳米颗粒（纳米金刚石，ND）在尤文肉瘤细胞中递送小干扰RNA（siRNA）的能力，从体内抗癌核酸药物递送的角度来看。将siRNA吸附到预先用阳离子聚合物包被的ND上。已经通过利用来自嵌入色心缺陷的ND固有荧光证明了ND的细胞摄取。这些包被的ND的细胞毒性显示为低。与内化功效一致，我们已经显示在含血清的培养基中通过ND载体化的siRNA在mRNA和蛋白质水平特异性抑制EWS/Fli-1基因表达。
英文摘要：
We investigated the ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) in Ewing sarcoma cells, in the perspective of in vivo anti-cancer nucleic acid drug delivery. siRNA was adsorbed onto NDs previously coated with cationic polymer. Cell uptake of NDs has been demonstrated by taking advantage of NDs intrinsic fluorescence coming from embedded color center defects. Cell toxicity of these coated NDs was shown to be low. Consistent with the internalization efficacy, we have shown a specific inhibition of EWS/Fli-1 gene expression at the mRNA and protein level by the ND vectorized siRNA in a serum containing medium.
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