海藻酸钠/磁性纳米粒子、壳聚糖-磁性纳米粒子和纤维素包覆磁性纳米粒子固定化酵母细胞在乙醇发酵中的应用
Application in the Ethanol Fermentation of Immobilized Yeast Cells in Matrix of Alginate/Magnetic Nanoparticles, on Chitosan-Magnetite Microparticles and Cellulose-coated Magnetic Nanoparticles
摘要：
以海藻酸钠和磁性纳米粒子为载体包埋酿酒酵母细胞，并将其共价固定在磁性壳聚糖和纤维素包覆的磁性纳米粒子上。制备了纤维素包覆的磁性纳米粒子和壳聚糖磁性纳米粒子，分别固定了耐热α-淀粉酶和糖化酶。将固定化细胞和酶应用于柱反应器中，1/用于用固定化葡糖淀粉酶同时进行玉米淀粉糖化，并用包埋或共价固定化酵母细胞生产乙醇，2/用于用固定化酵母单独进行淀粉水解产物的乙醇发酵。研究了固定化α-淀粉酶和葡萄糖淀粉酶对玉米淀粉的水解以及固定化α-淀粉酶对玉米淀粉的单独水解。在第一反应器中，乙醇产率达到约10%。理论值的91%;产率约为86%，第二。乙醇发酵受固定化类型、初始颗粒装载量、料糖浓度和稀释率的影响。在还原糖浓度为200.0 g/L、颗粒负载率为70%、稀释率为3.0 h-1的条件下，包埋细胞的乙醇产量可达264.0 g/L. h。所制备的固定有酵母细胞的磁性颗粒在4 ° C的盐水中稳定超过1个月。.
英文摘要：
Saccharomyces cerevisiae cells were entrapped in matrix of alginate and magnetic nanoparticles and covalently immobilized on magnetite-containing chitosan and cellulose-coated magnetic nanoparticles. Cellulose-coated magnetic nanoparticles with covalently immobilized thermostable α-amylase and chitosan particles with immobilized glucoamylase were also prepared. The immobilized cells and enzymes were applied in column reactors - 1/for simultaneous corn starch saccharification with the immobilized glucoamylase and production of ethanol with the entrapped or covalently immobilized yeast cells, 2/ for separate ethanol fermentation of the starch hydrolysates with the fixed yeasts. Hydrolysis of corn starch with the immobilized α-amylase and glucoamylase, and separate hydrolysis with the immobilized α-amylase were also examined. In the first reactor the ethanol yield reached approx. 91% of the theoretical; the yield was approx. 86% in the second. The ethanol fermentation was affected by the type of immobilization, the initial particle loading, feed sugar concentration and the dilution rate. The ethanol productivity with entrapped cells reached 264.0 g/L.h at particle loading rate 70% and dilution rate 3.0 h-1 with reducing sugar concentration of 200.0 g/L. The prepared magnetic particles with fixed yeast cells were stable at 4oC in saline for more than 1 month. .
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