基于光镊和视频显微镜技术的非桑蚕丝品种的微流变学研究
Microrheology of non mulberry silk varieties by optical tweezer and video microscopy based techniques
摘要：
我们已经进行了比较研究的丝素蛋白溶液的微观流变性能形成的各种丝绸土著印度次大陆。我们提出了测量的粘弹性模量的Tasar丝素蛋白溶液中使用单和双光镊在0.16%和0.25%（w/v）。使用光学镊子进行的测量的带宽扩展到较低的频率制度的视频显微镜测量。此外，我们已经测量了Eri和Muga品种的丝素蛋白溶液在较高浓度（1.00%w/v）下的粘弹性模量，将测量工具限制为视频显微镜，因为这些溶液在较高浓度下的降低的光学折射率排除了基于光镊的研究。选择后者丝品种的较高浓度的丝心蛋白溶液是为了能够比较从光学方法获得的剪切模量与从广角X射线散射数据获得的它们相应的纤维刚度。我们报告的丝素蛋白溶液的浓度研究这些丝绸品种的微观结构和微观流变参数之间的相关性。
英文摘要：
We have carried out a comparative study of the microrheological properties of silk fibroin solutions formed from a variety of silks indigenous to the Indian subcontinent. We present the measured viscoelastic moduli of Tasar silk fibroin solution using both a single and dual optical tweezer at 0.16% and 0.25% (w/v). The bandwidth of the measurements carried out using optical tweezers is extended down to the lower frequency regime by a video microscopy measurement. Further, we have measured the viscoelastic moduli of Eri and Muga varieties of silk fibroin solutions at a higher concentration (1.00% w/v) limiting the tool of measurement to video microscopy, as the reduced optical transparencies of these solutions at higher concentration preclude an optical tweezer based investigation. The choice of a higher concentration of fibroin solution of the latter silk varieties is so as to enable a comparison of the shear moduli obtained from optical methods with their corresponding fibre stiffness obtained from wide angle X-ray scattering data. We report a correlation between the microstructure and microrheological parameters of these silk varieties for the concentration of fibroin solutions studied.
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