光循环蛋白跨膜光传输的特征噪声特性
Characteristic noise features in light transmission across membrane protein undergoing photocycle
摘要：
我们展示了一种基于噪声测量的技术，该技术可用于研究蛋白质组装中的动力学过程。光刺激下细菌视紫红质（bR）等膜蛋白系统的动力学直接可视化是一项极具挑战性的工作。bR代表一个模型系统，其中刺激触发的结构动力学和生物功能直接相关。我们的方法利用泵浦-探测近场显微镜方法在传输模式，并涉及分析从一个有限大小的分子组装的透射率波动。概率密度分布表明有限的大小和统计相关性的影响出现作为一个特征频率分布的噪声光谱的bR，其起源可以追溯到光循环动力学。有价值的洞察到的分子过程中获得的噪音研究的bR和其突变体D96 N作为外部参数的函数，如温度，湿度或存在的一个额外的泵源。
英文摘要：
We demonstrate a technique based on noise measurements which can be utilized to study dynamical processes in protein assembly. Direct visualization of dynamics in membrane protein system such as bacteriorhodopsin (bR) upon photostimulation are quite challenging. bR represents a model system where the stimulus-triggered structural dynamics and biological functions are directly correlated. Our method utilizes a pump-probe near field microscopy method in the transmission mode and involves analyzing the transmittance fluctuations from a finite size of molecular assembly. Probability density distributions indicating the effects of finite size and statistical correlations appear as a characteristic frequency distribution in the noise spectra of bR whose origin can be traced to photocycle kinetics. Valuable insight into the molecular processes were obtained from the noise studies of bR and its mutant D96N as a function of external parameters such as temperature, humidity or presence of an additional pump source.
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