人类基因组序列的核苷酸含量、基因结构和基因表达特征之间的关系（PhD概要）
Relationships among the nucleotide content of human genome sequence, gene structure, and gene expression features (PhD synopsis)
摘要：
本学位论文主要研究人类基因组功能元件与其核苷酸结构之间的关联。选择核苷酸含量的不对称性（偏斜、偏差）作为核苷酸结构的主要特征。一个显着的差异，核苷酸含量的不对称性被发现人类外显子与内含子。具体地，外显子序列显示对嘌呤的偏好（即，A和G超过C和T），而内含子表现出酮-氨基偏斜（即G和T超过A和C）。这些偏差的程度取决于基因表达模式。最高的内含子酮-氨基偏斜被发现在管家基因的内含子中。在内含子的情况下，其序列处于弱修复系统下，AT->GC和CG->TA置换优先累积。比较分析了智人和代表性哺乳动物细胞色素P450 2 E1的基因序列。根据Cyp 2 e1基因编码序列的相似性，构建了Cyp 2 e1基因的分支树。开发了一种新的NCBI数据库序列挖掘和分析的编程工具，从而建立了自己的数据库。
英文摘要：
The Dissertation is focused on the studies of associations between functional elements in human genome and their nucleotide structure. The asymmetry in nucleotide content (skew, bias) was chosen as the main feature for nucleotide structure. A significant difference in nucleotide content asymmetry was found for human exons vs. introns. Specifically, exon sequences display bias for purines (i.e., excess of A and G over C and T), while introns exhibit keto-amino skew (i.e. excess of G and T over A and C). The extents of these biases depend upon gene expression patterns. The highest intronic keto-amino skew is found in the introns of housekeeping genes. In the case of introns, whose sequences are under weak repair system, the AT->GC and CG->TA substitutions are preferentially accumulated. A comparative analysis of gene sequences encoding cytochrome P450 2E1 of Homo sapiens and representative mammals was done. The cladistic tree on the basis of coding sequences similarity of the gene Cyp2e1 was constructed. A new programming tools of NCBI database sequence mining and analysis was developed, resulting in construction of a own database.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
