具有非平衡结合的Botts和Morales一般改性剂机理的动力学行为
Kinetic behavior of the general modifier mechanism of Botts and Morales with non-equilibrium binding
摘要：
在本文中，我们进行了一个完整的分析的动力学行为的一般改性剂机制的Botts和Morales在平衡稳态和非平衡稳态（NESS）。本文受马尔可夫链非平衡理论的启发，引入净通量的概念进行讨论，得到了NESS中的生产率表达式，具有明确的生物物理意义。到目前为止，人们普遍认为，作为活化剂或抑制剂是改性剂的固有性质。然而，我们发现，这种传统的观点是基于均衡假设。当反应体系不平衡时，改性剂可能不再是总的活化剂或抑制剂。根据修饰剂浓度对酶活性的调节作用，将修饰剂的动力学行为分为三类，分别命名为双曲线行为、钟形行为和开关行为。我们表明，开关现象，其中一个改性剂可以转换之间的活化剂和抑制剂的改性剂浓度变化时，只发生在NESS。以药物对Pgp ATP酶活性的影响为例，说明了这种转换现象的发生。
英文摘要：
In this paper, we perform a complete analysis of the kinetic behavior of the general modifier mechanism of Botts and Morales in both equilibrium steady states and non-equilibrium steady states (NESS). Enlightened by the non-equilibrium theory of Markov chains, we introduce the net flux into discussion and acquire an expression of product rate in NESS, which has clear biophysical significance. Up till now, it is a general belief that being an activator or an inhibitor is an intrinsic property of the modifier. However, we reveal that this traditional point of view is based on the equilibrium assumption. A modifier may no longer be an overall activator or inhibitor when the reaction system is not in equilibrium. Based on the regulation of enzyme activity by the modifier concentration, we classify the kinetic behavior of the modifier into three categories, which are named hyperbolic behavior, bell-shaped behavior, and switching behavior, respectively. We show that the switching phenomenon, in which a modifier may convert between an activator and an inhibitor when the modifier concentration varies, occurs only in NESS. Effects of drugs on the Pgp ATPase activity, where drugs may convert from activators to inhibitors with the increase of the drug concentration, are taken as a typical example to demonstrate the occurrence of the switching phenomenon.
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