利用光吸收直接探测单分子
Direct detection of single molecules by optical absorption
摘要：
单分子光学的出现对从生物物理学到材料科学、光物理学和量子光学等领域产生了深远的影响。然而，所有现有的室温单分子方法都是基于高效发射体的荧光检测。在这里，我们证明了标准的，从传统的吸收光谱仪已知的无调制测量确实可以检测到单个分子。我们报告在环境条件下，即使在黑暗的状态下，例如在光闪烁或强淬灭的单分子的吸收截面的定量测量。我们的工作将单分子显微镜和光谱学扩展到一大类吸收光但不能有效发荧光的材料。
英文摘要：
The advent of single molecule optics has had a profound impact in fields ranging from biophysics to material science, photophysics, and quantum optics. However, all existing room-temperature single molecule methods have been based on fluorescence detection of highly efficient emitters. Here we demonstrate that standard, modulation-free measurements known from conventional absorption spectrometers can indeed detect single molecules. We report on quantitative measurements of the absorption cross section of single molecules under ambient condition even in their dark state, for example during photoblinking or strong quenching. Our work extends single-molecule microscopy and spectroscopy to a huge class of materials that absorb light but do not fluoresce efficiently.
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