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arXiv 1007.0065physics.opticsphysics.bio-ph
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A novel approach for structure analysis of two-dimensional membrane protein crystals using x-ray powder diffraction data

Ruben A. Dilanian, Connie Darmanin, Jose N. Varghese, Steve W. Wilkins, Toshihiko Oka, Naoto Yagi, Harry M. Quiney, Keith A. Nugent

英文摘要:

The application of powder diffraction methods in two-dimensional crystallography is regarded as intractable because of the uncertainties associated with overlapping reflections. Here, we report an approach that resolves these ambiguities and provides reliable low-resolution phase information directly from powder diffraction data. We apply our method to the recovery of the structure of the bacteriorhodopsin (bR) molecule to a resolution of 7 angstroms using only powder diffraction data obtained from two-dimensional purple membrane (PM) crystals.

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