细胞骨架在PC12神经突体积调节和珠状转变中的作用
The role of the cytoskeleton in volume regulation and beading transitions in PC12 neurites
摘要：
我们目前的调查体积调节和串珠形状的转变，在PC12神经突进行使用流动室技术。通过用特定的药物破坏细胞骨架，我们研究了其单个组分在体积调节反应中的作用。我们发现，微管破坏增加肿胀率和达到的最大体积，但不影响神经突恢复其初始体积的能力。此外，对轴突串珠化（也称为珍珠状不稳定性）的研究为神经突的机械状态提供了额外的线索。我们的结论是，最初的溶胀阶段是机械减慢微管，而体积恢复是由被动扩散的渗透压。我们的实验提供了一个框架，以调查体积内稳态的细胞骨架力学的作用。
英文摘要：
We present investigations on volume regulation and beading shape transitions in PC12 neurites conducted using a flow-chamber technique. By disrupting the cell cytoskeleton with specific drugs we investigate the role of its individual components in the volume regulation response. We find that microtubule disruption increases both swelling rate and maximum volume attained, but does not affect the ability of the neurite to recover its initial volume. In addition, investigation of axonal beading --also known as pearling instability-- provides additional clues on the mechanical state of the neurite. We conclude that the initial swelling phase is mechanically slowed down by microtubules, while the volume recovery is driven by passive diffusion of osmolites. Our experiments provide a framework to investigate the role of cytoskeletal mechanics in volume homeostasis.
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