囊泡中小叶组成不对称性的无标记光谱检测
Label-free, Spectroscopic Detection of Leaflet Composition Asymmetry in Vesicles
摘要：
振动和频散射（SFS）已被用来研究亚微米，阴阳离子囊泡在溶液中。囊泡合成从十二烷基三甲基溴化铵（DTAB）和十二烷基硫酸钠（SDS）表面活性剂在氘代水的二元混合物，自发组装成热稳定的囊泡。这些囊泡的稳定性归因于内外双层小叶之间的表面活性剂浓度不对称。这种浓度不对称性应该是由SFS观察到的，由于本地反转破缺。在1044 cm处观察到对应于SDS头基的对称硫酸盐拉伸模式的信号，表明小叶的局部结构确实存在不对称性。结果表明，它应该是可能的，以测量脂质体在水溶液中的界面结构和研究原位过程，如糖和蛋白质的结合，这是重要的许多过程中的生物物理化学。
英文摘要：
Vibrational sum frequency scattering (SFS) has been used to study sub-micron, catanionic vesicles in solution. The vesicles were synthesized from a binary mixture of dodecyltrimethylammonium bromide (DTAB) and sodium dodecylsulfate (SDS) surfactants in deuterated water, which spontaneously assemble into thermodynamically stable vesicles. The stability of these vesicles is attributed to a surfactant concentration asymmetry between the inner and outer bilayer leaflets. This concentration asymmetry should be observable by SFS due to local inversion symmetry-breaking. Signal corresponding to the symmetric sulfate stretch mode of the SDS head group is observed at 1044 cm, indicating that there is indeed asymmetry in the local structure of the leaflets. The results indicate that it should be possible to measure the interfacial structure of liposomes in aqueous solution and study in-situ processes like the binding of sugars and proteins that are important for many processes in biophysical chemistry.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
