噬菌蛭弧菌对大肠杆菌双种群生物膜的捕食作用coli Prey和M.黄体诱饵
Bdellovibrio bacteriovorus Predation in Dual-Species Biofilms of E. coli Prey and M. luteus Decoys
摘要：
生物膜是生长在界面处的基质封闭的微生物群落。它们表现出显着的表型变化，使它们对许多抗菌剂具有抗性，这些抗菌剂可以杀死它们自由游泳的同行。环境中的生物膜可以由单个或多个微生物物种组成。该组先前的研究表明，噬菌蛭弧菌（一种革兰氏阴性捕食性细菌，消耗其他革兰氏阴性细菌）可以在某些条件下根除单一物种的大肠杆菌生物膜1。这些结果提示了B.在工业、医疗和环境背景下生物膜根除中的食菌性细菌。本文研究了噬菌蛭弧菌与多菌种生物膜，特别是双菌种生物膜相互作用和清除的可能性。coli（革兰氏阴性菌）和M.黄体（革兰氏阳性诱饵）在液体和空气之间的界面。我们研究了这种双物种生物膜是否对B更敏感、更敏感或同样敏感。bacteriovorus攻击相比，其单一物种的同行。利用不同的研究方法，包括细菌培养，细胞计数，结晶紫染色，革兰氏染色，光学显微镜，扫描电子显微镜和原子力显微镜，以了解蛭弧菌与生物膜的相互作用。实验表明，B. bacteriovorus不仅消耗E. coli，但也减弱了M.黄体的固体表面，使生物膜易于去除。B.噬菌不仅控制猎物，还控制周围介质中的诱饵细菌种群，后者可能通过竞争营养物。这些实验促使我们思考B.可以使用噬菌菌体来控制环境中的生物膜。
英文摘要：
Biofilms are matrix-enclosed microbial communities that grow at interfaces. They exhibit significant phenotypic changes that render them resistant to many antibacterial agents that can kill their free-swimming counterparts. Biofilms in the environment can consist of either a single or multiple microbial species. Previous investigations in this group demonstrated that Bdellovibrio bacteriovorus, a gram-negative predatory bacterium that consumes other gram-negative bacteria, could eradicate a single-species E.coli biofilm in some conditions1. These results suggest the potential value of B. bacteriovorus in biofilm eradication in industrial, medical and environmental contexts. Here we carried out investigation of the potential of Bdellovibrio bacteriovorus to interact with and remove multi-species biofilms, specifically dual-species biofilms of E. coli (gram-negative prey) and M. luteus (gram-positive decoy) at interfaces between liquid and air. We examined whether this kind of dual-species biofilms is more, less, or equally susceptible to B. bacteriovorus attack compared to their single-species counterparts. Different research methods including bacterial culture, cell counting, crystal violet staining, gram staining, optical microscopy, scanning electron microscopy, and atomic force microscopy were exploited to gain an insight into Bdellovibrio's interaction with biofilms. Our experiments showed that B. bacteriovorus not only consumes E. coli but also weakens the attachment of M. luteus to the solid surface, rendering the biofilms susceptible to removal. B. bacteriovorus keeps in check not only prey but also decoy bacterial populations in the surrounding media, the latter probably via competition for nutrients. These experiments encourage us to consider how B. bacteriovorus might be used to control biofilms in the environment.
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