基于碳纳米管拉曼标记的癌细胞和肿瘤组织近红外多色分子成像
Multiplexed five-color molecular imaging of cancer cells and tumor tissues with carbon nanotube Raman tags in the near-infrared
摘要：
单壁碳纳米管（SWNTs）与五个不同的C13/C12同位素组成和良好分离的拉曼峰已被合成和共轭的五个靶向配体，以赋予分子特异性。活细胞的多重拉曼成像已经通过用单壁碳纳米管的五色混合物对细胞进行高度特异性染色来进行。肿瘤样品的离体多路拉曼成像揭示了从细胞培养到体内肿瘤生长的LS 174 T结肠癌细胞上表皮生长因子受体（EGFR）的令人惊讶的上调。这是第一次在单一激光激发下在近红外（NIR）区域进行五色多路复用分子成像。由于纳米管特有的尖锐拉曼峰超过了生物物种的低、平滑的自发荧光背景，实现了成像的近零干扰背景。
英文摘要：
Single-walled carbon nanotubes (SWNTs) with five different C13/C12 isotope compositions and well-separated Raman peaks have been synthesized and conjugated to five targeting ligands in order to impart molecular specificity. Multiplexed Raman imaging of live cells has been carried out by highly specific staining of cells with a five-color mixture of SWNTs. Ex vivo multiplexed Raman imaging of tumor samples uncovers a surprising up-regulation of epidermal growth factor receptor (EGFR) on LS174T colon cancer cells from cell culture to in vivo tumor growth. This is the first time five-color multiplexed molecular imaging has been performed in the near-infrared (NIR) region under a single laser excitation. Near zero interfering background of imaging is achieved due to the sharp Raman peaks unique to nanotubes over the low, smooth autofluorescence background of biological species.
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