镍离子与多核苷酸及不同GC组成的DNA的相互作用
Nickel (II) Ions Interaction with Polynucleotides and DNA of Different GC Composition
摘要：
这项工作的目的是研究GC替代二聚体在DNA与Ni（II）离子结合中的作用。采用紫外差光谱法研究了Ni（Ⅱ）离子与产气荚膜梭菌、小鼠肝（C3 HA系）、小牛胸腺、鲑鱼精子、鲱鱼精子、大肠杆菌、藤黄微球菌等组织中DNA及多聚核苷酸Poly（dA-dT）xPoly（dA-dT）、Poly（dG）xPoly（dC）、Poly（dG-dC）xPoly（dG-dC）的相互作用。结果表明，Ni（II）离子在大沟一侧与DNA双螺旋的外球结合选择了更稳定的二聚体3 ^'-C-G-5^'。. 5 ^'-G-C-3^'并与二聚体中两个鸟嘌呤的N7原子结合，形成G-G链间交联。它直接与沃森-克里克错对型点缺陷（产生罕见的酮-烯醇和氨基-亚氨基互变异构形式）和脱嘌呤化的形成过程相关。
英文摘要：
The goal of the work was to study the role of GC alternative dimmers in the binding of DNA with Ni (II) ions. The method of ultraviolet difference spectroscopy has been applied to investigate Ni (II) ions interactions with DNA extracted from Clostridium perfringens, Mice liver (C3HA line), Calf thymus, Salmon sperm, Herring sperm, E.coli, Micrococcus luteus and polynucleotides Poly (dA-dT)xPoly (dA-dT), Poly (dG)x Poly (dC), Poly (dG-dC)xPoly (dG-dC). It is shown that Ni (II) ions at outer-spherical binding with DNA double helix from the side of the major groove choose more stable dimmers 3^'-C-G-5^' . . 5^'-G-C-3^' and get bound with N7 atoms of both guanines in dimmer forming G-G interstrand crosslink. It directly correlates to the process of forming point defects of Watson-Crick wrong pair type (creation of rare keto-enolic and amino-imino tautomeric forms) and depurinization.
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