生化反应中细胞间变异的宏观动力学效应
Macroscopic Kinetic Effect of Cell-to-Cell Variation in Biochemical Reactions
摘要：
在相同的环境条件下，遗传上相同的细胞可以显示出蛋白质拷贝数的强烈变化，这是由于个体细胞中固有的随机事件。在这里，我们开发了一个理论框架，以解决酶丰度的变化如何影响在细胞群体内观察到的代谢反应的集体动力学。在细胞群体水平上测量的动力学参数被证明是系统偏离单细胞，即使在均匀参数的群体。由于这些考虑，Michaelis-Menten动力学甚至可能不适合应用于群体水平。我们的研究结果阐明了体内和体外动力学之间的差异的一个新的起源，并提供了潜在的效用，分析单细胞代谢组学数据。
英文摘要：
Genetically identical cells under the same environmental conditions can show strong variations in protein copy numbers due to inherently stochastic events in individual cells. We here develop a theoretical framework to address how variations in enzyme abundance affect the collective kinetics of metabolic reactions observed within a population of cells. Kinetic parameters measured at the cell population level are shown to be systematically deviated from those of single cells, even within populations of homogeneous parameters. Because of these considerations, Michaelis-Menten kinetics can even be inappropriate to apply at the population level. Our findings elucidate a novel origin of discrepancy between in vivo and in vitro kinetics, and offer potential utility for analysis of single-cell metabolomic data.
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