基于石墨烯的薄片的荧光猝灭显微镜可视化
Visualizing Graphene Based Sheets by Fluorescence Quenching Microscopy
摘要：
基于石墨烯的片材由于其优异的机械、电学和热学性能而引起了极大的兴趣。允许快速观察这些单原子层的通用可视化方法将是非常期望的，因为它可以极大地促进样品评估和操作，并提供即时反馈以改进合成和处理策略。在这里，我们报告说，石墨烯基片可以通过淬灭染料涂层的发射在荧光显微镜下高度可见，染料涂层可以方便地通过冲洗去除，而不会破坏片。目前用于石墨烯基片的成像技术依赖于使用特殊基底。相反，荧光猝灭机制不再受底物类型的限制。沉积在任意基底上的石墨烯、还原的氧化石墨烯或甚至氧化石墨烯片现在可以容易地通过眼睛可视化，具有用于层计数的良好对比度。还证明了在溶液中悬浮片的直接观察。荧光淬灭显微镜提供了前所未有的成像灵活性，并可能成为表征石墨烯基材料的通用工具。
英文摘要：
Graphene based sheets have stimulated great interest due to their superior mechanical, electrical and thermal properties. A general visualization method that allows quick observation of these single atomic layers would be highly desirable as it can greatly facilitate sample evaluation and manipulation, and provide immediate feedback to improve synthesis and processing strategies. Here we report that graphene based sheets can be made highly visible under a fluorescence microscope by quenching the emission from a dye coating, which can be conveniently removed afterwards by rinsing without disrupting the sheets. Current imaging techniques for graphene based sheets rely on the use of special substrates. In contrast, the fluorescence quenching mechanism is no longer limited by the types of substrates. Graphene, reduced graphene oxide, or even graphene oxide sheets deposited on arbitrary substrates can now be readily visualized by eye with good contrast for layer counting. Direct observation of suspended sheets in solution was also demonstrated. The fluorescence quenching microscopy offers unprecedented imaging flexibility and could become a general tool for characterizing graphene based materials.
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