荧光碳纳米粒子的合成、表征及生物成像应用
Fluorescent Carbon Nanoparticle: Synthesis, Characterization and Bio-imaging Application
摘要：
采用硝酸氧化炭黑的方法合成了粒径为2-6 nm的荧光碳纳米颗粒（CNP），量子产率约为3%，该方法可用于毫克级水溶性纳米颗粒的合成。这些CNP是具有主要石墨结构的纳米晶体，并且在UV暴露下显示绿色荧光。虽然硝酸氧化诱导氮和氧结合到烟灰颗粒中，提供水溶性和发光性质;从不同尺寸颗粒的混合物中分离小颗粒提高了荧光量子产率。这些CNP显示了令人鼓舞的细胞成像应用。它们进入细胞而没有任何进一步的功能化，并且这些颗粒的荧光性质可以用于基于荧光的细胞成像应用。
英文摘要：
Fluorescent carbon nanoparticle (CNP) having 2-6 nm in size with quantum yield of about ~3% were synthesized via nitric acid oxidation of carbon soot and this approach can be used for milligram scale synthesis of these water soluble particles. These CNPs are nano-crystalline with predominantly graphitic structure and shows green fluorescence under UV exposure. While nitric acid oxidation induces nitrogen and oxygen incorporation into soot particle that afforded water solubility and light emitting property; the isolation of small particles from a mixture of different size particles improved the fluorescence quantum yield. These CNP shows encouraging cell imaging application. They enter into cell without any further functionalization and fluorescence property of these particles can be used for fluorescence based cell imaging application.
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