质量传感BioCD蛋白芯片的临床应用--前列腺特异性抗原检测
Mass-sensing BioCD Protein Array towards Clinical Application: Prostate Specific Antigen Detection in Patient Sera
摘要：
用于蛋白质检测的质量传感生物传感器阵列不需要荧光团或酶标记。然而，很少有质量生物传感器蛋白质阵列已被证明成功应用于高背景样品，如血清。在本文中，我们测试的BioCD作为一个质量生物传感器的基础上，通过希夫碱还原共价连接的抗体的光学干涉。我们使用BioCD在96孔抗PSA微阵列中检测患者血清中的前列腺特异性抗原（PSA，前列腺癌的生物标志物）。我们在全血清中达到了4 ng/ml的检测限，并测量了三名患者血清中的PSA浓度。
英文摘要：
Mass-sensing biosensor arrays for protein detection require no fluorophores or enzyme labels. However, few mass biosensor protein arrays have demonstrated successful application in high background samples, such as serum. In this paper, we test the BioCD as a mass biosensor based on optical interferometry of antibodies covalently attached through Schiff-base reduction. We use the BioCD to detect prostate specific antigen (PSA, a biomarker of prostate cancer) in patient sera in a 96-well anti-PSA microarray. We have attained a 4 ng/ml detection limit in full serum and have measured PSA concentrations in three patient sera.
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