人红细胞的最佳通量状态、反应可替换性和对基因敲除的反应
Optimal flux states, reaction replaceability and response to knockouts in the human red blood cell
摘要：
表征基因组尺度代谢网络的能力、临界性和对扰动的响应是具有重要应用的基本问题。一个关键的问题涉及到潜在的最有害的基因敲除的识别。组合方法与采样技术的整合，以探索可行的通量状态的空间可能会提供关键的见解在这个问题上。我们通过列举从底物到产物的替代途径来评估人红细胞中每种代谢转化的可替代性，从而获得单一酶病潜在损害的完整图谱。对健康细胞和突变细胞中的最佳通量状态的空间进行采样，揭示了拓扑和动力学方面之间的相关性和互补性。
英文摘要：
Characterizing the capabilities, criticalities and response to perturbations of genome-scale metabolic networks is a basic problem with important applications. A key question concerns the identification of the potentially most harmful knockouts. The integration of combinatorial methods with sampling techniques to explore the space of viable flux states may provide crucial insights on this issue. We assess the replaceability of every metabolic conversion in the human red blood cell by enumerating the alternative paths from substrate to product, obtaining a complete map of the potential damage of single enzymopathies. Sampling the space of optimal flux states in the healthy and in the mutated cell reveals both correlations and complementarity between topologic and dynamical aspects.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
