功能性亚10 nm铈和铁氧化物颗粒在细胞培养基中的电空间增强稳定性
Electrosteric enhanced stability of functional sub-10 nm cerium and iron oxide particles in cell culture medium
摘要：
纳米颗粒在生物学中的应用要求纳米颗粒在含有高浓度蛋白质和盐的溶液中以及在细胞培养基中保持稳定。在这项工作中，我们开发了简单的方案，用于涂覆亚10 nm纳米颗粒，并评估了分散体在各种环境中的胶体稳定性。配体（柠檬酸），低聚物（膦酸酯封端的聚（环氧乙烷））和聚合物（聚（丙烯酸））被用作纳米厚的吸附层的铈（CeO 2）和铁（γ-Fe 2 O3）的氧化物纳米粒子。有机官能团通过物理（静电）力吸附在颗粒表面上。通过静态和动态光散射进行高离子强度和细胞培养基中的稳定性试验。在所检查的三种涂层中，我们发现只有聚（丙烯酸）在长期（>数周）内完全保持分散稳定性。提高的稳定性解释的多点连接的链到颗粒表面上，并通过adlayer介导的电空间相互作用。这些结果表明，带阴离子电荷的聚合物代表了传统涂层剂的有效替代品。
英文摘要：
Applications of nanoparticles in biology require that the nanoparticles remain stable in solutions containing high concentrations of proteins and salts, as well as in cell culture media. In this work, we developed simple protocols for the coating of sub-10 nm nanoparticles and evaluated the colloidal stability of dispersions in various environments. Ligands (citric acid), oligomers (phosphonate-terminated poly(ethylene oxide)) and polymers (poly(acrylic acid)) were used as nanometer-thick adlayers for cerium (CeO2) and iron (gamma-Fe2O3) oxide nanoparticles. The organic functionalities were adsorbed on the particle surfaces via physical (electrostatic) forces. Stability assays at high ionic strength and in cell culture media were performed by static and dynamic light scattering. Among the three coating examined, we found that only poly(acrylic acid) fully preserved the dispersion stability on the long term (> weeks). The improved stability was explained by the multi-point attachments of the chains onto the particle surface, and by the adlayer-mediated electrosteric interactions. These results suggest that anionically charged polymers represent an effective alternative to conventional coating agents.
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