非进行性肌球蛋白马达滑行试验中肌动蛋白丝的旋转运动
Twirling motion of actin filaments in gliding assays with non-processive myosin motors
摘要：
我们提出了一个模型研究的滑翔测定中，肌动蛋白丝移动的非进行性肌球蛋白马达。我们表明，即使动力蛋白的动力行程没有横向分量，细丝将围绕其轴旋转，同时在表面上移动。值得注意的是，这种旋转运动的旋向与肌动蛋白丝结构的旋向相反。这是因为滑动的肌动蛋白丝有“靶区”，在那里它的亚基指向表面，因此更容易被肌球蛋白头接近。每个肌球蛋白头在到达靶区中心之前比之后具有更高的结合概率，这导致左旋旋转。我们提出了一个随机模拟和近似的解析解。计算的旋转运动的节距取决于细丝速度（ATP浓度）。它在低速时达到约400 nm，并随着速度的增加而增加。
英文摘要：
We present a model study of gliding assays in which actin filaments are moved by non-processive myosin motors. We show that even if the power stroke of the motor protein has no lateral component, the filaments will rotate around their axis while moving over the surface. Notably, the handedness of this twirling motion is opposite from that of the actin filament structure. It stems from the fact that the gliding actin filament has "target zones" where its subunits point towards the surface and are therefore more accessible for myosin heads. Each myosin head has a higher binding probability before it reaches the center of the target zone than afterwards, which results in a left-handed twirling. We present a stochastic simulation and an approximative analytical solution. The calculated pitch of the twirling motion depends on the filament velocity (ATP concentration). It reaches about 400nm for low speeds and increases with higher speeds.
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