冬青栎（Quercus ilex L.）在西西里
Narrow genetic base in forest restoration with holm oak (Quercus ilex L.) in Sicily
摘要：
为了实证评估实际种子取样策略对西西里岛冬青栎林遗传多样性〈动〉<动>的影响，我们利用6个核微卫星位点分析了两个实生批（苗木和人工林）及其已知天然种子起源林的遗传组成。结果表明，与原始林分相比，实生苗群体的遗传多样性〈动〉性<动>显著降低，遗传组成差异显著。通过幼苗的母体分配和等位基因频率的时间变化来量化亲本的雌性和总有效数量。估计的有效生育数极低（Nfe $\approx$ 2-4），考虑种子和花粉供体的估计值也很低（Ne $\approx$ 35-50）。这些价值观可以解释为一个不适当的林业种子收获策略限于少数空间接近的树木。
英文摘要：
In order to empirically assess the effect of actual seed sampling strategy on genetic diversity of holm oak (Quercus ilex) forestations in Sicily, we have analysed the genetic composition of two seedling lots (nursery stock and plantation) and their known natural seed origin stand by means of six nuclear microsatellite loci. Significant reduction in genetic diversity and significant difference in genetic composition of the seedling lots compared to the seed origin stand were detected. The female and the total effective number of parents were quantified by means of maternity assignment of seedlings and temporal changes in allele frequencies. Extremely low effective maternity numbers were estimated (Nfe $\approx$ 2-4) and estimates accounting for both seed and pollen donors gave also low values (Ne $\approx$ 35-50). These values can be explained by an inappropriate forestry seed harvest strategy limited to a small number of spatially close trees.
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