生物细胞后向散射中的光谱振荡
Spectral oscillations in backscattering of light from a biological cell
摘要：
从上皮生物细胞的弹性光后向散射实验观察到的节拍样信号的可能来源进行了鉴定。拍频样信号的来源最有可能是细胞核，它是一种球形颗粒，具有光学锐边、低相对折射率和共振范围内的尺寸参数;另一个可能的来源是类似尺寸的细胞器的聚集。利用Mie理论对具有类生物核参数的电介质球进行了计算机模拟，结果表明，后向散射作为尺寸参数的函数具有类拍模式。高频周期振荡是单次散射的贡献，而多次散射事件给出缓慢变化的振荡贡献。随着对比度的增加，后向散射信号从纯正弦曲线变为拍频信号。低对比度粒子散射中的拍状图案不依赖于球体的任何特定特征;在具有尖锐光学对比度和光滑形状的星形非球形物体的散射中可以观察到类似的图案;在这种情况下，振荡的高频率取决于物体相对于入射光的取向。
英文摘要：
Possible origins of the experimentally observed beat-like signal in elastic light backscattering from an epithelial biological cell are identified. The source of the beat-like signal is most likely the cellular nucleus, which is a spheroidal particle with an optically sharp edge, low relative index of refraction, and a size parameter in the resonant range; another possible source is an agglomeration of cell organelles of a similar size. Using Mie theory computer simulations for a dielectric sphere with biological nucleus-like parameters, we have shown that backscattering as a function of size parameter has a beat-like pattern. The high frequency periodic oscillation is the single scattering contribution, while multiple scattering events give slowly varying oscillatory contributions. With increasing contrast, the backscattering signal changes from a pure sinusoid to that of a beat signal. The beat-like pattern in scattering from a low contrast particle does not depend on any particular features of the sphere; a similar pattern is observable in scattering from star-like non-spherical objects with a sharp optical contrast and smooth shape; in this case, the high frequency of oscillations depends on the object orientation relative to incident light
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
