用加压板张力计精确测量液体和组织的表面张力
Measuring accurately liquid and tissue surface tension with a compression plate tensiometer
摘要：
表观组织表面张力允许量化细胞-细胞凝聚力，并且据报道是胚胎发育期间发生的细胞重排或癌症进展的有力指标。测量是利用毛细管定律用平行压缩板张力计实现的。虽然它是在十多年前引入的，但它是基于各种几何或物理近似的。令人惊讶的是，这些近似值从未被测试过。使用一种新的张力计，我们比较了目前使用的两种方法来测量组织表面张力，并提出了第三个，基于局部多项式拟合（LPF）的压缩液滴或细胞聚集体的配置文件。我们显示的重要性，测量板和滴/聚集体之间的接触角，以获得真正准确的测量表面张力时，应用现有的方法。我们可以怀疑，许多报道的表面张力值受到很大影响，因为没有正确处理这个参数。然后，我们使用新引入的LPF方法，这是不依赖于这个参数的好处。这些发现证实了通过生成数字压缩液滴配置文件和测试的鲁棒性和不同的方法的误差的敏感性。
英文摘要：
Apparent tissue surface tension allows the quantification of cell-cell cohesion and was reported to be a powerful indicator for the cellular rearrangements that take place during embryonic development or for cancer progression. The measurement is realized with a parallel compression plate tensiometer using the capillary laws. Although it was introduced more than a decade ago, it is based on various geometrical or physical approximations. Surprisingly, these approximations have never been tested. Using a novel tensiometer, we compare the two currently used methods to measure tissue surface tension and propose a third one, based on a local polynomial fit (LPF) of the profile of compressed droplets or cell aggregates. We show the importance of measuring the contact angle between the plate and the drop/aggregate to obtain real accurate measurement of surface tension when applying existing methods. We can suspect that many reported values of surface tension are greatly affected because of not handling this parameter properly. We show then the benefit of using the newly introduced LPF method, which is not dependent on this parameter. These findings are confirmed by generating numerically compressed droplet profiles and testing the robustness and the sensitivity to errors of the different methods.
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