核糖体RNA转录的随机性和交通堵塞：终止和抗终止的有趣作用
Stochasticity and traffic jams in the transcription of ribosomal RNA: Intriguing role of termination and antitermination
摘要：
在快速生长的细菌中，核糖体RNA（rRNA）需要以非常高的速率转录以维持对核糖体合成的高细胞需求。这导致RNA聚合酶（RNAP）的密集运输。我们开发了一个随机模型，整合了单分子和定量体内研究的结果E。coli中，以评估暂停、终止和反终止对rRNA转录的定量影响。我们的计算表明，在密集的RNAP交通，自发暂停RNAP可能会导致严重的“交通堵塞”，表现在广泛的分布间RNAP距离，可以是一个主要因素限制转录，因此增长。我们的研究结果表明，抑制这些暂停的核糖体抗终止复合物是必不可少的快速增长。此外，即使是几个非antiterminated RNAP的未抑制暂停已经可以大大减少密集流量下的转录。然而，终止因子Rho可以去除非抗终止的RNAP并恢复快速转录。因此，结果表明Rho增强而不是减弱rRNA转录的有趣作用。
英文摘要：
In fast growing bacteria, ribosomal RNA (rRNA) is required to be transcribed at very high rates to sustain the high cellular demand on ribosome synthesis. This results in dense traffic of RNA polymerases (RNAP). We developed a stochastic model, integrating results of single-molecule and quantitative in vivo studies of E. coli, to evaluate the quantitative effect of pausing, termination, and antitermination on rRNA transcription. Our calculations reveal that in dense RNAP traffic, spontaneous pausing of RNAP can lead to severe "traffic jams", as manifested in the broad distribution of inter-RNAP distances and can be a major factor limiting transcription and hence growth. Our results suggest the suppression of these pauses by the ribosomal antitermination complex to be essential at fast growth. Moreover, unsuppressed pausing by even a few non-antiterminated RNAPs can already reduce transcription drastically under dense traffic. However, the termination factor Rho can remove the non-antiterminated RNAPs and restore fast transcription. The results thus suggest an intriguing role by Rho to enhance rather than attenuate rRNA transcription.
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