细胞在粘附之前如何在粘附表面上踮起脚尖
How cells tiptoe on adhesive surfaces before sticking
摘要：
细胞膜上布满了突起，这些突起用电子显微镜进行了彻底的分析。然而，细胞膜产生的纳米级三维运动以适应外来表面的地形并启动粘附仍然知之甚少。在这里，我们描述的动态表面变形显示的单核细胞碰撞对纤连蛋白涂层表面。我们观察到典型的5 nm振幅和5-10秒的寿命的膜波动。细胞膜表现为微米尺度的独立单元。细胞在50 nm分离处检测到外来表面的存在，导致膜波动的时间依赖性放大。分子接触，然后随之而来的明显的细胞膜分离30-40 nm，并在随后的几十秒内，这一距离稳步下降。接触成熟与大分子的平面内排出和强大的膜波动有关。因此，膜的起伏可能是细胞对底物形貌的敏感性、相互作用的结果和接触延伸的初始动力学的主要决定因素
英文摘要：
Cell membranes are studded with protrusions that were thoroughly analyzed with electron microscopy. However, the nanometer-scale three-dimensional motions generated by cell membranes to fit the topography of foreign surfaces and initiate adhesion remain poorly understood. Here, we describe the dynamics of surface deformations displayed by monocytic cells bumping against fibronectin-coated surfaces. We observed membrane undulations with typically 5 nm amplitude and 5-10 second lifetime. Cell membranes behaved as independent units of micrometer size. Cells detected the presence of foreign surfaces at 50 nm separation, resulting in time-dependent amplification of membrane undulations. Molecular contact then ensued with apparent cell-membrane separation of 30-40 nm, and this distance steadily decreased during the following tens of seconds. Contact maturation was associated with in-plane egress of bulky molecules and robust membrane fluctuations. Thus, membrane undulations may be the major determinant of cell sensitivity to substrate topography, outcome of interaction and initial kinetics of contact extension
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
