单链DNA在固体基质上的固定化
Immobilization of single strand DNA on solid substrate
摘要：
基于层层自组装技术的薄膜可用于将DNA固定到固体载体上。本文报道了用聚阳离子聚烯丙胺盐酸盐（PAH）通过静电相互作用将DNA固定在固体载体上。紫外-可见吸收光谱和稳态荧光光谱的研究表明，DNA在LbL膜中的组织特征。最重要的观察是，单链DNA被固定在PAH骨干的LbL膜时，膜制造的DNA的熔化温度以上。当温度恢复到室温时，以这种方式固定在LbL膜上的DNA保持原样，并且即使在几天后组织也保持不受影响。紫外-可见吸收光谱研究证实了这一发现。
英文摘要：
Thin films based on Layer-by-Layer (LbL) self assembled technique are useful for immobilization of DNA onto solid support. This communication reports the immobilization of DNA onto a solid support by electrostatic interaction with a polycation Poly (allylamine hydrochloride) (PAH). UV-Vis absorption and steady state fluorescence spectroscopic studies exhibit the characteristics of DNA organized in LbL films. The most significant observation is that single strand DNA are immobilized on the PAH backbone of LbL films when the films are fabricated above the melting temperature of DNA. DNA immobilized in this way on LbL films remains as such when the temperature is restored at room temperature and the organization remains unaffected even after several days. UV-Vis absorption spectroscopic studies confirm this finding.
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