低pH浓度下牛血清白蛋白与阴离子接枝共聚物库仑作用形成可溶性纳米粒子的表征
Characterization of the Soluble Nanoparticles Formed through Coulombic Interaction of Bovine Serum Albumin with Anionic Graft Copolymers at Low pH
摘要：
两种阴离子接枝共聚物与牛血清白蛋白混合物的静态光散射研究（丙烯酸钠-co-2-丙烯酰胺基-2-甲基-1-丙磺酸钠）接枝聚（N，N-二甲基丙烯酰胺），在聚（N，N-二甲基丙烯酰胺）（PDMAM）侧链，揭示了在pH低于4.9时形成带相反电荷的复合物，BSA的等电点。对在pH = 3.00下形成的核-冠纳米颗粒进行了表征。通过SLS测定了它们的分子量和回转半径，而通过动态光散射测定了它们的流体动力学半径。使用小角中子散射测量来确定不溶性络合物的半径，所述不溶性络合物包括颗粒的核心。结果表明，中性PDMAM侧链上接枝共聚物的含量越高，纳米粒子的尺寸和聚集数越小。如果要使用胃肠道，这样的颗粒应该是令人感兴趣的药物递送候选物。
英文摘要：
A static light scattering (SLS) study of bovine serum albumin (BSA) mixtures with two anionic graft copolymers of poly (sodium acrylate-co-sodium 2-acrylamido-2-methyl-1-propanesulphonate)-graft-poly (N, N-dimethylacrylamide), with a high composition in poly (N, N-dimethylacrylamide) (PDMAM) side chains, revealed the formation of oppositely charged complexes, at pH lower than 4.9, the isoelectric point of BSA. The core-corona nanoparticles formed at pH = 3.00, were characterized. Their molecular weight and radius of gyration were determined by SLS, while their hydrodynamic radius was determined by dynamic light scattering. Small angle neutron scattering measurements were used to determine the radius of the insoluble complexes, comprising the core of the particles. The values obtained indicated that their size and aggregation number of the nanoparticles, were smaller when the content of the graft copolymers in neutral PDMAM side chains was higher. Such particles should be interesting drug delivery candidates, if the gastrointestinal tract was to be used.
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