液体环境中细胞和生物分子系统的局部机电探测
Towards local electromechanical probing of cellular and biomolecular systems in a liquid environment
摘要：
机电耦合在生物系统中普遍存在，例如钙化和结缔组织中的简单压电性，电压门控离子通道，线粒体中的能量储存，以及心肌细胞和外毛细胞静纤毛中的机电活动。压电响应力显微镜（PFM）最初是作为一种研究铁电材料中机电现象的技术出现的，近年来，已被用于研究广泛的非铁电极性材料，包括压电生物材料。同时，该技术已从环境扩展到模型铁电系统上的液体成像。在这里，我们提出了几个模型的细胞和生物分子系统，包括胰岛素和溶菌酶淀粉样纤维，乳腺癌细胞和细菌视紫红质在液体环境中的局部机电探测的结果。SPM在液体中的操作的具体功能划定和瓶颈的路线走向纳米分辨率的机电成像的生物系统被确定。
英文摘要：
Electromechanical coupling is ubiquitous in biological systems with examples ranging from simple piezoelectricity in calcified and connective tissues to voltage-gated ion channels, energy storage in mitochondria, and electromechanical activity in cardiac myocytes and outer hair cell stereocilia. Piezoresponse force microscopy (PFM) has originally emerged as a technique to study electromechanical phenomena in ferroelectric materials, and in recent years, has been employed to study a broad range of non-ferroelectric polar materials, including piezoelectric biomaterials. At the same time, the technique has been extended from ambient to liquid imaging on model ferroelectric systems. Here, we present results on local electromechanical probing of several model cellular and biomolecular systems, including insulin and lysozyme amyloid fibrils, breast adenocarcinoma cells, and bacteriorhodopsin in a liquid environment. The specific features of SPM operation in liquid are delineated and bottlenecks on the route towards nanometer-resolution electromechanical imaging of biological systems are identified.
机构、总结、中文摘要均由AI分析而来，可能存在错误，请以论文正文为准。
