环状DNA-蛋白质复合物体内行为的多级解构
Multilevel Deconstruction of the In Vivo Behavior of Looped DNA-Protein Complexes
摘要：
具有环的蛋白质-DNA复合物在从DNA转录的调节到端粒维持的各种细胞过程中发挥重要作用。尽管它们无处不在，但它们的精确体内特性及其整合到细胞功能中仍然在很大程度上未被探索。在这里，我们提出了一个多层次的方法，有效地连接在两个方向的分子特性与细胞生理学，并使用它来表征的环状DNA-lac阻遏物复合物的分子特性，而在体内发挥作用。我们发现的属性包括存在的两个代表性的构象的复杂的，稳定的一个构象的DNA建筑蛋白质，和精确的值的基本扭转弹性常数和弯曲自由能。将所有这些分子信息整合到基因调控模型中，揭示了环状DNA-蛋白质复合物在塑造基因表达特性方面前所未有的多功能性。
英文摘要：
Protein-DNA complexes with loops play a fundamental role in a wide variety of cellular processes, ranging from the regulation of DNA transcription to telomere maintenance. As ubiquitous as they are, their precise in vivo properties and their integration into the cellular function still remain largely unexplored. Here, we present a multilevel approach that efficiently connects in both directions molecular properties with cell physiology and use it to characterize the molecular properties of the looped DNA-lac repressor complex while functioning in vivo. The properties we uncover include the presence of two representative conformations of the complex, the stabilization of one conformation by DNA architectural proteins, and precise values of the underlying twisting elastic constants and bending free energies. Incorporation of all this molecular information into gene-regulation models reveals an unprecedented versatility of looped DNA-protein complexes at shaping the properties of gene expression.
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